This pilot study investigated levels of burnout in non-clinical workers in a healthcare setting and compared them to salivary cortisol concentrations. Mashlach’s Burnout Inventory-Human Service Survey for Medical Personnel (MBI-HSS(MP)) was used to evaluate self-perceived levels of burnout. Saliva samples were collected to measure cortisol concentrations at four times during participants’ shift. The study included subjects exposed to multiple high-stress factors such as working in a healthcare setting in post-COVID-19 conditions at a major medical center in Southwest Montana. The results revealed a significant correlation (P-value < 0.05) between morning salivary cortisol secretion concentrations and mean survey scores. Significant differences were also found between the three dimensions measured by the MBI-HSS(MP): burnout, depersonalization, and personal achievement, p-value < 0,05. The pilot study has several limitations that should be considered when interpreting results. Findings do suggest that future investigation among this group is warranted. Results may help guide interventions that support the mental health and wellbeing of medical support personnel, reduce stress, and avoid burnout.