Fig. 1. (A) Relative abundance of OC-I transcripts in the leaves of three individual plants of the OCI-1 (OCI-1/1, OCI-1/2, OCI-1/3) and OCI-2 (OCI-2/1, OCI-2/2, OCI-2/ 3) lines measured under HN conditions. The dotted line indicates the level of transcripts encoding OCI-related endogenous soybean cystatins, which were also amplified by the primer pair applied for OCI amplification. This basal value was set at 1. Bars are means ± SD of three experiments. (B) Cathepsin L-like cysteine protease activities measured in cotyledons. Error bars indicate mean ± SD of three biological replicates derived from leaf material measured in triplicate.

Published as part of Quain, Marian D., Makgopa, Matome E., Cooper, James W., Kunert, Karl J. & Foyer, Christine H., 2015, Ectopic phytocystatin expression increases nodule numbers and influences the responses of soybean (Glycine max) to nitrogen deficiency, pp. 179-187 in Phytochemistry 112 (1) on page 182, DOI: 10.1016/j.phytochem.2014.12.027, http://zenodo.org/record/10487581