Fig. 4. SDS–PAGE of the CW proteins of the maize roots after in vitro collagenase digestion. The isolated and purified CW proteins were denatured or not and then collagenase-digested for 1 or 12 h at 37 °C. After the SDS–PAGE separation, the proteins were stained with SYPRO Ruby. C: control CW proteins without digestion; —: without denaturation; +: after denaturation; coll— and coll+: CW proteins after collagenase digestion without and after denaturation, respectively; coll: pure enzyme.

Published as part of Luczak, Magdalena, Krzeszowiec-Jeleń, Weronika, Konopka-Postupolska, Dorota & Wojtaszek, Przemysław, 2015, Collagenase as a useful tool for the analysis of plant cellular peripheries, pp. 195-209 in Phytochemistry 112 (1) on page 198, DOI: 10.1016/j.phytochem.2014.11.013, http://zenodo.org/record/10487515