Fig. 2. Structure-based sequence alignment of the crystallized M1PDH from Shigella flexneri (Sh._flexneri) (PDB code 3H2Z) with orthologues from different organisms. The ESPript3 program (Robert and Gouet, 2014) was used to highlight the conserved amino acids which are shown in white letters on a red background. The consensus sequence is shown below the sequence alignment: amino acid strictly conserved in columns are noted in uppercase letters, while those conserved in more than 50% of the aligned sequences are noted as lowercase letters. The secondary structure of the Sh. flexneri protein is shown above the alignment. Alpha-helices are represented as helices and β- turns as arrows. Five conserved blocks as defined by Klimacek et al. (2003) for PSLDRs, named A to E, are represented below the conserved consensus sequence. M1PDH GenBank accession numbers are: Shigella flexneri (Sh._flexneri) KFZ96362; Bacillus subtilis subsp. subtilis str. 168 (Bac._subtilis), CAB12207; Enterococcus faecalis V583 (En._faecalis), NP_814201; E. coli (E._coli) str. K-12 substr. MG1655, NP_418057; Klebsiella pneumoniae (K._pneumoniae), AAD45386; Vibrio cholerae O395 (V._cholera), YP_001215036; Beauveria bassiana (B._bassiana), ACU32784; Alternaria alternata (A._alternata), AAQ63948; Phaeosphaeria nodorum SN15 (P._nodorum), Q0U6E8; Aspergillus fumigatus Af293 (As._fumigatus), XP_755399; Aspergillus niger (As._niger), AAL89587; Ei. tenella (Ei._tenella), O96437; EsMPDH1, D7FMJ8; EsMPDH2, D8LIO9; EsMPDH3, D7G793; Saccharina japonica (S._japonica), (Deng et al., 2012); Micromonas pusilla (M._pusilla), C1N0F1; Micromonas sp. (Micromonas_sp.), C1FJR6.