Fig. 7. Amidohydrolase activity (µmol min–1 mg of protein–1) of purified FAAH protein with different [1-14C]N-acylethanolamines (NAEs) in presence of 100 µM of N-isopropyllauroylamide (3) or N-ethyllauroylamide (4). Reactions were initiated by the addition of purified FAAH (0.3 µg) in 50 mM Bis-Tris propane–HCl (pH 9.0) and different radiolabeled NAEs (12:0, 16:0 and 20:4) in a final volume of 0.15 ml. Reactions proceeded at 30 °C, 30 min, with shaking (120 rpm). Data points represent means ± S.D. of triplicate assays. Plots were generated with SigmaPlot software version 12.0. p-Value of <0.05 is indicated by * as determined by Student's t test. ns, not significant.