Fig. 3. Phylogenetic analysis of the amino acid sequences of steroid C-22 hydroxylases. This analysis involved 16 homologs from the following species: Os (Oryza sativa japonica group), Sl (Solanum lycopersicum), Pe (Populus euphratica), Gh (Gossypium hirsutum), Mt (Medicago truncatula), Cc (Cajanus cajan), Ep (Echinacea purpurea), At (Arabidopsis thaliana), Zm (Zea mays), Pp (Paris polyphylla), Vc (Veratrum californicum), St (Solanum tuberosum), Ar (Ajuga reptans), and Bm (Bombyx mori). The PpCYP90B29 from this study is highlighted by a red diamond shape. The evolutionary analyses were conducted in MEGA6 using the neighbor-joining method, and the percentage of replicate trees in which the associated taxa clustered together among the 1000 replicates in the bootstrap test are shown next to the branches. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Published as part of Yin, Yan, Gao, Linhui, Zhang, Xianan & Gao, Wei, 2018, A cytochrome P450 monooxygenase responsible for the C-22 hydroxylation step in the Paris polyphylla steroidal saponin biosynthesis pathway, pp. 116-123 in Phytochemistry 156 on page 119, DOI: 10.1016/j.phytochem.2018.09.005, http://zenodo.org/record/10484444