Fig. 1. Multiple alignment, phylogenetic analysis, and organ expression analysis of TaHQT1 and TaHQT2. a. Sequence alignment of HQTs sequences in T.antungese. Multiple sequence alignment of TaHQT1 (in this article, MK033609), TaHQT2 (MH683053), CcHQT1 (ADL62854.1), CcHQT2 (ACJ23164.1), NtHQT (CAE46932.1), and CiHQT (ANN12610.1) The conserved domain PLN02663 (HXXXD) and pfam02458 (DFGWG) is indicated by a straight line. Black boxes indicate the conserved structural motifs in the acyl-transferase family. b. Phylogenetic analysis of hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase. The phylogenetic tree was constructed using MEGA software (version7.0) based on the neighbour-joining method. Values above the branches are bootstrap percentages (1000 replicates). Abbreviations and gene ID were as follows. TaHQT1 (this article, MK033609), Taraxacum antungense hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase 1; TaHQT2 (MH683053), Taraxacum antungense hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase 2; CiHQT(ANN12610.1) Cichorium intybus hydroxycinnamoyl-CoA quinate/shikimate hydroxycinnamoyl transferase; CcHQT2 (ACJ23164.1), Cynara cardunculus var. scolymus hydroxycinnamoyl CoA quinate transferase 2; CcHQT1(ADL62854.1), Cynara cardunculus var. scolymus hydroxycinnamoyl-CoA transferase 1; PgHQT(AEM63676.1), Platycodon grandiflorus hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase; LjHQT (ACZ52698), L.japonica HQT; LmHQT1(ALJ56099.1), Lonicera macranthoides HQT1; SlHQT(CAE46933.1), Solanum lycopersicum hydroxycinnamoyl CoA quinate transferase; StHQT (NP_001275483.1), Solanum tuberosum HQT; NtHQT (CAE46932.1), N. tabacum HQT; The TaHQTs protein as labeled after taxon marker. c. Organ expression of TaHQT1 and TaHQT2 gene in wild T.antungese (leave, root, flower, and scape, respectively). Different letters indicate significant differences in comparisons (P <0.05).