This dataset includes multiplex immunofluorescence images (mostly 3+1 channels) of frozen human kidney sections that have been used to validate a panel of 27 antibodies and 1 lectin designed to define the main cellular and extracellular matrix (ECM) comparments in the normal human kidney. We have attached an excel file (Supplemental Table 1) that includes all of the experimental and (de-identified) patient metadata associated with these images with information, and comments about each of the images, the antibodies used, and the cell types and ECM compoartments identified using these antibodies. Because of the size and number of images used for these studies, we have divided this into two separate datasets (Antibody validation studies Parts 1 and 2). A subset of these antibodies have also been evaluated for both 2D and 3D cyclical immunofluorescence studies that have been included in separate datasets under this umbrella "community". These are identified in the "Antibodies used" tab in Supplemental Table 1 (CyCIF Cycles).