A Simulated Heterozygous Diploid Genome For Third-Gen Sequencing, Assembly, And Curation

A simulated heterozygous diploid genome based on Saccharomyces cerevisiae, and S. paradoxus homologous chromosomes. Simulated PacBio subreads were generated from both parent haplomes and mixed together. A phased assembly was produced using FALCON assembler and FALCON Unzip (doi:10.1038/nmeth.4035). This dataset and assembly were then used to validate the Purge Haplotigs pipeline (https://bitbucket.org/mroachawri/purge_haplotigs). See workflow.sh for commands, comments and file descriptions.

Related Organizations

Australian Wine Research Institute
Australia

Keywords

Purge Haplotigs

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