The objective of this document is to list the guidelines for the implementation of standardized methods of microfluidic components. These guidelines will primarily concentrate on creating effective port connections between the macroscale world and the microscale fluidic channels, while also addressing crucial issues connected to alterations in flow dynamics and pressure. This report is based on input from A3.3.1 - A3.3.4. Activity number: A3.3.5 M28 Activity description: Using input from A3.3.1-A3.3.4, IMTAG with support from INESC MN, TUBITAK, LNE, CEA, CETIAT and EnablingMNT will produce guidelines for the implementation of standardised methods of microfluidic components focusing on port connection from microscale fluidic channels to the macroscale world andassociated changes in flow and pressure. The guidelines will be submitted to standardisation groups such as ISO/TC48/WG3 and WG5, ISO/TC229, CEN/TC332/WG7.Once agreed by the consortium, the coordinator on behalf of IMTAG, INESC MN, TUBITAK, LNE, CEA, CETIAT and EnablingMNT will submit the guidelines to EURAMET as D6: “Guidelines for the implementation of standardised methods of microfluidic components focusing on port connection from microscale fluidic channels to the macroscale world and associated changes in flow and pressure”. Partners (lead in bold): IMTAG, INESC MN, TUBITAK, EnablingMNT, CEA, CETIAT, LNE