As the first action by the Inter-EURLs Working Group on Next Generation Sequencing, a survey was conducted across the NRLs in order to acquire knowledge on the level of adoption and the status of the capacity towards the use of such methodologies across the NRLs networks, in order to define the activities of the WG and to target the actions of the EURLs on the actual needs of the NRLs, by considering specific needs in the different networks to be addressed through combined and harmonised actions by the EURLs in the WG.