This dataset is underlying the scientific publication titled "Enhanced Susceptibility to Tomato Chlorosis Virus (ToCV) in Hsp90- and Sgt1-Silenced Plants: Insights from Gene Expression Dynamics", published in the Viruses journal. The dataset includes a time-course transcriptome analysis using RNA-seq of naïve (no whitefly and no virus), mock (non-viruliferous whiteflies) and ToCV (ToCV_viruliferous whiteflies)-treated tomato samples at 2, 7, and 14 days post-infection (dpi) and viral small RNAs derived from Tomato plants infected with ToCV at 14 dpi. The dataset provided here has been deposited in full by the authors in the European Nucleotide Archive (ENA) at EMBL-EBI under accession number PRJEB67704 (https://www.ebi.ac.uk/ena/browser/view/PRJEB67704The provided information in the dataset are further discussed and interpreted in detail, as well as their subsequent results, in the scientific publication. This research was conducted within the VIRTIGATION project, which is part of the EU Open Research Data pilot. This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No. 101000570.