Each experiment was conducted on the Multipad Agarose Plate (MAP) and is labeled with BE followed by a number. This repository contains four main elements: The file Experiment condition map.json describes how the MAP platforms were set up for each experiment. Some datasets also include data that was discarded. The folder Benchmarking contains data for how we validated the performance of our PadAnalyser package. Originals have a set of images of bacteria affected by antibiotics that produce different morphologies. Annotated_raw is the human-labelled version of these, and Annotated are these converted to a format that can be used by code for comparison. Segmentation Benchmarking.ipynb is a Jupyter Notebook that segments the cells and compares the output to the annotated data. The folder Dataframes contains Pandas data frames from all experiments, classified by bacteria species. The folder Videos contains debug videos visualising how the segmentation algorithm performed on the different datasets. Contact us for access to the raw data. Videos in the format *_ssi.mp4 contain single-cell masks, and *_css.mp4 contains colony masks.