One of the human pathogens that spreads the most is Staphylococcus aureus. Because of the damage it does to life and, by extension, the economy, it became imperative to ascertain its prevalence and antibiogram in our surroundings in order to implement appropriate management and treatment. Records of microbiological cultures and outcomes of antibiotic sensitivity testing for possible UTI cases. The results led to the conclusion that every S. aureus isolate tested positive for the clfA gene. The results showed resistance to Benzylpenicillin 100%, oxacillin 100%, gentamicin 40%, tobramycin 40%, levofloxacin 80%, erythromycin 100%, clindamycin 100%, trimethoprim-sulfamethoxazole 60%, and tetracycline 60% acid at 80%. In other words, Staphylococcus aureus in UTI is resistant to most antibiotics, and the best antibiotic used during the study, bacteria sensitive to it is gentamicin, which is considered the lowest percentage of resistance compared to the rest of the antibiotics used during the current study. Keywords: Staphylococcus0aureus, VITEK, Antibiotic, Resistance, clfA