The present experiment was evaluated to study of the effect of Consciousness Energy Healing Treatment (the Trivedi Effect ® ) on novel proprietary test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of vitamin D3 metabolites in kidney tissue. A novel proprietary test formulation was formulated with a few minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as the untreated test formulation, while the other part of the test formulation and the animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. 25-OH vitamin D 3 level was significantly increased in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treatment per se (G6), and 15-days pre-treatment of the Biofield Energy Treated test formulation (G7) groups by 21%, 22.9%, and 11.5%, respectively as compared with the disease control group (G2). Additionally, the level of 1,25-(OH) 2 D 3 was increased by 55% in the G6 group as compared with the G2. Further, the expression of CYP24A in kidney was increased by 13.9% in the G6 group, while CYP27B expression in kidney was significantly increased by 17.6%, 18.6%, 10.1%, and 15.6% in the G5, G6, G7 (Biofield Energy Treated test formulation from day -15), and G8 (Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15) groups, respectively as compared with the G4 group. Vitamin D receptor (VDR) expression was significantly increased by 69.5% and 31% in the G6 and G7 groups, respectively as compared with the G2 group. Overall, the results showed significant improvement of the availability of vitamin D 3 metabolites, that could be helpful for the vitamin D 3 deficiency persons to maintain good bone health and immunity. Therefore, the results suggest that there was significant slowdown in the disease progression and disease related all other complications/symptoms in the Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups ( viz . G6, G7, G8, and G9) comparatively with the disease group.