The present study focused the determine structural chemistry of the otoliths of red porgy, Pagrus pagrus, by using Raman analysis. The Raman analysis was conducted within the spectral scanning range of 3785-50 cm-1. According to the Raman spectrum of otoliths, it becomes evident that there are two significant vibration bands that contribute to the otolith's main structure. The first is the intense vibration band at 1095 cm-1, known as the symmetric (V1) band of the carbonate (-CO3-2) molecule. The second is the moderate in-plane bending band at 710 cm-1, referred to as the in-plane (V4) band. These bands play a fundamental role in the structure of the otolith. Additionally, the Raman spectrum of the otolith reveals the presence of bands related to metal bonds forming with the carbonate (-CO3-2) molecule. These bands are observed at 155 and 210 cm-1 and exhibit a moderate intensity. However, it is important to note that the specific cations or metal bonds involved cannot be determined solely from these bands. The Raman analysis of red porgy sagittal otolith composition and structure has provided valuable insights into the chemical composition and structure of these fish ear stones. The application of Raman spectroscopy to examine otoliths seems like a highly useful technique for understanding the composition and structure of these crystals. By utilizing Raman spectroscopy, researchers can gain valuable insights into the chemical composition and structures of otoliths in the inner ear. This examination allows for the determination of the minerals and elements contained within otoliths.