Neurodegenerative diseases are among the leading cause of non-fatal disease burden in India. One in seven Indians was affected by neurodegenerative diseases. Although neurodegenerative agents is used for the management of neurodegenerative diseases like anxiety, psychosis, depression and mania it may side effects such as tardative dyskinesia, weight gain, weight loss, muscle cramps, dysphoria, gastrointestinal upset, eye problems or problems with blood tests. In this present review we made an exposure regarding many screening methods on evaluation of neurodegenerative diseases like Alzheimer's disease (AD), Parkinson's disease (PD), Amyotrophic lateral sclerosis, Huntington's disease, and Friedreich's ataxia, in the aspects of preclinical studies. These methods are very useful to evaluate the neurodegenerative agents like anti- Alzheimer's agents, anti- Parkinson's agents. Keywords: neurodegenerative diseases, preclinical studies, anti- Alzheimer's agents, anti- Parkinson's agents