Personalised medicine (PM) research seeks to identify interventions that can be targeted to individual patients based on their predicted response. Efficient advancement of PM depends on the availability of validated patient-targeted biomarkers. In order to deliver them, we need a more precise understanding of the molecular profiles of individuals, which is facilitated by multi-omics approaches. To turn the multi-omics promises into a reality, systemic bottlenecks impacting the biomarker field need to be overcome. As part of the EATRIS-Plus project, we developed the Multi-omics Toolbox (MOTBX), to support the translational medicine research community by providing validated resources, such as standardised protocols for individual omics performance and analysis, quality, and data management guidelines, for the development, the implementation and the adoption of multi-omics approaches in real PM. The MOTBX is a resource that has been built with, and for the community. This means that researchers are not only welcome to use the MOTBX, but to support its further development by actively contributing with valuable resources.