
In this report, five commercial antibodies against TATA-binding protein associated factor 2N (TAF15) were characterized for use Western Blot, immunoprecipitation, and immunofluorescence, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work is part of the ALS-Reproducible Antibody Platform (ALS-RAP), established as a public-private partnership by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
antibody characterization, 68 kDa TATA-binding protein-associated factor, Western Blot, immunoprecipitation, RNA-binding, Q92804, DNA-binding, TATA-binding factor 2N, immunoblot, TAF15, RNA-binding protein 56, immunofluorescence, antibody validation, TATA-binding protein-associated factor 2N
antibody characterization, 68 kDa TATA-binding protein-associated factor, Western Blot, immunoprecipitation, RNA-binding, Q92804, DNA-binding, TATA-binding factor 2N, immunoblot, TAF15, RNA-binding protein 56, immunofluorescence, antibody validation, TATA-binding protein-associated factor 2N
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