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Abstract The present work is devoted to the assessment of mycological safety according to conditional pathogenic fungus in work place urban ecosystem. It was determined that the composition of dominant nucleus of aeromycobiotic system directly formed in urban ecosystems depends on the humidity of the urban environment. In addition, it was revealed that if the humidity in the urban environment is more than 70%, the number of conditional pathogen fungus inside the aeromycobiota is rising and the risk factor among the population is increasing.