ABSTRACT The presentstudy was aimed at investigating the cardioprotactive activities of the Gossypin was isolated from Hibiscus vitifolius by methanolic extract. The cardioprotactive activities of extract have been evaluated by using A portion of heart tissue was preserved in 10% formalin (pH 7.2) and subjected to histopathological studies .The heart sections were made 2mm thick and infarction area was evaluated by 2,3,4-Triphenyltetrazolium chloride(TTC) staining and biochemical analysis such as lactate dehydrogenase (LDH), creatine kinase (CK-MB), thiobarbituric acid reactive substances (TBARS), (GSH),(SOD),CAT were estimated in serum and heart of experimental animals using standard biochemical kits. Normal group showed normal cardiac fibre arrangements without necrotic fibres. ISO group showed intense necrosis, damage and irregular arrangements and morphological disturbances of myofibrils with increased interfibre distance. While standard (Losartan20mg/kg) and treated groups showed significant reduction in the damage produce by ISO group. The heart homogenate from the control group showed significant reduced activities of, SOD and increase activity of LPO compared to normal group. The Gossypin treated 5, 10, 20mg/kg groups showed significant protection by reducing elevated levels of LPO and increase level of antioxidant enzymes SOD , the non-enzymatic antioxidant GSH significantly as compared to control group. The blood serum from control group showed significant increase in enzyme marker LDH level as compared to normal groups. In contrast Gossypin (5, 10,20mg/kg) treated groups and standard group animals showed significant reduction in serum LDH level as compared to control. The blood serum from control group showed significant increase in enzyme marker LDH level as compared to normal groups. In contrast Gossypin (5, 10,20mg/kg) treated groups and standard group animals showed significant reduction in serum LDH level as compared to control. The results show the Cardioprotactive activity of Gossypin. Keywords: Cardioprotactive activity, Gossypin, Hibiscus vitifolius