This report guides researchers to select the most appropriate antibodies for Solute Carrier Family 12 Member 5. We used an antibody characterization pipeline based on knockout overexpression (KOOE) and knockout (KO) cells to perform head-to-head comparisons of commercial antibodies for SLC12A5 by immunoblot (Western blot) and immunofluorescence. HuH 7 SLC12A5 KO and KOOE cell lines are available at RESOLUTE and were used for antibody screening. In order to assess the correct overexpression of SLC12A5 (coupled to HA-Tag) under doxycycline induction in KOOE cell lines, anti-HA-Tag antibody was utilized. The authors do not provide an assessment of the quality of the tested antibodies as their respective performances are limited to our finite experimental conditions. The readers should interpret the present findings based on their own scientific expertise. The authors acknowledge that an antibody that demonstrates specificity in the stated test conditions can be suboptimal in adifferent experimental format or in cell lines that differ from those directly tested here.