Wild Triticeae grasses serve as important gene pools for forages and cereal crops. Knowledge on their ge-nome compositions is pivotal for efficient utilization of this vast gene pool in germplasm enhancement programs. Using DNA sequences of genome-specific RAPD markers, seleeted primers have been designed todevelop sequence tagged site (STS) markers. Genome specificity was lost for a majority of RAPD-to-STSconversions due to the inward extension of primer sequences. However, successful eonversion has beenachieved for genomes E', E, St, H, Ns, W, V and Y (an unknown genome in many polyploid Elymus spe-cies). Several cleaved amplified polymorphic sequence (CAPS) markers were also developed to distinguishthe E', E and R genomes. The identified STS and CAPS markers are useful in suggesting the presence ofcertain genome(s) in Triticeae species and in identifying the alien chromosome or chromosomal segment inwheat addition, substitution, or translocation lines. Use of STS markers has helped to identify wheat add-tion lines with W- and Y-genome chromosomes derived from hybrids of hexaploid wheat Triicum aestivumn(AABBDD) and hexaploid Elymus rectisetus (StStWWYY). Seven disomie wheat addition lines having different Egenome chromosomes are eonfirmed by the CAPS markers for this genome. This study also pro-vides evidence that barley yellow dwarf virus (BYDV) resistant germplasm lines from Purdue and Chinaare different those developed in Australia.