Background: Ankle injury is common for soccer players. The number of peripheral blood stem cells (PBSC), is a key parameter in the extent of recovery. In animal studies, StemEnhance® shows accelerated muscle repair after induced muscle injury, and shown to trigger a significant increase in the number of PBSC. Determine whether SE2® could enhance the effectiveness of conventional treatment for ankle injuries. Methods: Twelve male soccer players were randomly assigned, to either experimental (SE2®) or control group. Both groups received conventional treatment. The experimental group received 12 capsules of SE2® daily for a month. The team’s doctor evaluated patients with soft tissue sonograms at days 1, 7, 14 and 21. The Karlsson Peterson Scoring for Ankle function was taken every other day. The acute ankle injury was similar in both groups. The participants were matched. The SE2® group experienced a significantly reduction in pain, range of motion and joint stability compared with control group. Recovery time was on average 17 days for the SE2® group versus 21 days for the control group. The use of the stem cell mobilizer SE2® in conjunction with conventional treatment reduced the recovery time from acute ankle injuries in male football players.