Enbody_Behavioral_Ecology_2018_Testosterone_Raw_DataRaw testosterone data (plasma testosterone levels) to be processed by Androgens_Comparative.Rmd.Enbody_WSFW_androgens.csvEnbody_Behavioral_Ecology_2018_Experimental_Response_Mount_DataRaw data for analyzing response to simulated territorial intrusions by White-shouldered Fairywrens (Malurus alboscapulatus). Can be processed by Mount_Analysis_Revised.Rmd.Enbody_WSFW_mount_responses_revised_renamed.csvEnbody_Behavioral_Ecology_2018_Experimental_Response_YesOrNo_Mount_DataData for analyzing if White-shouldered Fairywrens responded to simulated territorial intrusions (binary 1 or 0). To be processed by Mount_Analysis_Revised.Rmd.binresp2.csvEnbody_Behavioral_Ecology_2018_Rscript_Testosterone_DataAn R markdown file (easily opened in RStudio) for analyzing raw testosterone data (in Enbody_WSFW_androgens.csv). Requires local installation of several R packages.Androgens_Comparative.RmdEnbody_Behavioral_Ecology_2018_RScript_Experimental_Response_Mount_DataAn R Markdown file (easily opened in R studio) for analyzing response to simulated territorial intrusions by White-shouldered Fairywrens (data in Enbody_WSFW_mount_responses_revised_renamed.csv and binresp2.csv).Mount_Analysis_Revised.RmdEnbody_RStudio_ProjectThis zip file contains the R Studio project that holds all the scripts and data that are also separately uploaded here on Dryad. You can just open this and run it out of the box, without downloading the other files. However, you will still need to install the R packages that are loaded.Enbody_WSFW_2018_Behavioral_Ecology.zip

In males, testosterone plays a key role in ornament production and linking ornamentation with reproductive behaviors and other traits to produce an integrated phenotype. Less is known about whether females couple testosterone, ornamentation, and aggressive behaviors to achieve female-specific combinations of traits. Ornamentation in females may be the result of correlated expression with male ornamentation, or female traits could arise as the result of sex specific selection pressures. Resolving between these alternatives is necessary to understand the degree to which selection acts on female traits. The White-shouldered Fairywren (Malurus alboscapulatus) provides a useful context to address these questions because populations vary in degree of female ornamentation, a derived trait, whereas male ornamentation is constant across both populations. We found that ornamented females have higher levels of circulating testosterone and respond more aggressively to experimental territorial intrusions than do unornamented females. These findings are consistent with the idea that, among female White-shouldered Fairywrens, testosterone may mechanistically link plumage and behavioral traits to produce an integrated competitive phenotype, as has been reported for males of closely related species. In contrast, circulating testosterone in males did not differ significantly between populations. More broadly, our findings are consistent with ongoing selection on the mechanisms underlying female ornaments, likely via social selection.