Downloads provided by UsageCountsData from: Ligation of glycophorin A generates reactive oxygen species leading to decreased red blood cell function
Khoory, Joseph; Estanislau, Jessica; Elkhal, Abdallah; Lazaar, Asmae; Melhorn, Mark I.; Brodsky, Abigail; Illigens, Ben; +6 Authors
Khoory, Joseph; Estanislau, Jessica; Elkhal, Abdallah; Lazaar, Asmae; Melhorn, Mark I.; Brodsky, Abigail; Illigens, Ben; Hamachi, Itaru; Kurishita, Yasutaka; Ivanov, Alexander R.; Shevkoplyas, Sergey; Shapiro, Nathan I.; Ghiran, Ionita C.;
Data from: Ligation of glycophorin A generates reactive oxygen species leading to decreased red blood cell function
Flow Cytometry Data of Sepsis StudyFlow cytometry data including C3d, C4d, glycocalyx (WGA), and some other data (FRAP, etc.)Flow Cytometry Data.xlsxM606 E0 + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M606 E0Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M606 E24 + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M606 E24Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M613E24-control C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control C3d.011M613E24-control C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control C4d.009M613E24-control DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control DAF.005M613E24-control IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control IgG.007M613E24-control RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control RBC + WGA.002M613E24-control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control RBC.001M613E24-sepsis C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis C3d.012M613E24-sepsis C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis C4d.010M613E24-sepsis DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis DAF.006M613E24-sepsis IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis IgG.008M613E24-sepsis RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC + WGA.004M613E24-sepsis RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC.003M613E48-control C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control C3d.013M613E48-control C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control C4d.012M613E48-control Ca 2+Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control Ca 2+.006M613E48-control IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control IgG.009M613E48-control NOFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control NO.007M613E48-control RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control RBC + WGA.003M613E48-control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control RBC.001M613E48-sepsis C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis C3d.014M613E48-sepsis C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis C4d.011M613E48-sepsis Ca 2+Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis Ca 2+.005M613E48-sepsis IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis IgG.010M613E48-sepsis NOFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis NO.008M613E48-sepsis RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC + WGA.004M613E48-sepsis RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC.002M616E0-normal RBC + c3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.normal RBC + c3d.004M616E0-normal RBC + c4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.normal RBC + c4d.005M616E0-normal RBC + IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.normal RBC + IgG.003M616E0-normal RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.normal RBC + WGA.002M616E0-Untreated normal RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Untreated normal RBC.001M616E0 RBC + c3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis M616E0 RBC + c3d.009M616E0 RBC + c4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis M616E0 RBC + c4d.010M616E0 RBC + IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis M616E0 RBC + IgG.008M616E0 RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis M616E0 RBC + WGA.007M616E0 RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis M616E0 RBC.006M616E24-Control RBC +IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC +IgG.005M616E24-Control RBC+C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC+C3d.007M616E24-Control RBC+C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC+C4d.009M616E24-Control RBC+DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC+DAF.011M616E24 RBC+C4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24 RBC+DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24 RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24.003M616E24+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24RBC +IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24RBC+C3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M616E24-Normal Control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Normal Control RBC.001M616E24-Normal Control RBC+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Normal Control RBC+WGA.002M618E0-Control C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C3D.006M618E0-Control C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C4D.007M618E0-Control DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control DAF.008M618E0-Control IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control IgG.005M618E0-Control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC.001M618E0-Control RBC+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC+WGA.002M618E0-Sepsis +DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis +DAF.012M618E0-Sepsis C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis C3D.010M618E0-Sepsis C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis C4D.011M618E0-Sepsis IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis IgG.009M618E0-Sepsis RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC.003M618E0-Sepsis RBC+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC+WGA.004M618E24-Data1Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.001M618E24-Data2Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.002M618E24-Data3Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.003M618E24-Data4Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.004M618E24-Data5Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.005M618E24-Data6Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.006M618E24-Data7Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.007M618E24-Data8Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.008M618E24-Data9Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.009M618E24-Data10Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.010M618E24-Data11Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.011M618E24-Data12Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Data.012M625E0-Control C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C3D.004M625E0-Control C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C4D.005M625E0-Control DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control DAF.006M625E0-Control IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control IgG.003M625E0-Control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC.001M625E0-Control RBC+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC+WGA.002M625E0-Sepsis RBC C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC C3D.010M625E0-Sepsis RBC C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC C4D.011M625E0-Sepsis RBC DAFFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC DAF.012M625E0-Sepsis RBC IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC IgG.009M625E0-Sepsis RBC E0Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC E0.007M625E0-Sepsis RBC E0+WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Sepsis RBC E0+WGA.008M625E24-control c3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control c3d.011M625E24-control c4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control c4d.012M625E24-control IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control IgG.009M625E24-control NOFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.control NO.010M625E24-Control RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC + WGA.002M625E24-Control RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control RBC.001M625E24-sepsis c3dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis c3d.008M625E24-sepsis c4dFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis c4d.006M625E24-sepsis NOFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis NO.007M625E24-sepsis RBC + WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC + WGA.004M625E24-sepsis RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis RBC.003M625E24-sepsis IgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.sepsis IgG.005M630E24-Control C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C3D.004M630E24-Control C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Control C4D.005M798E0 Ctrl C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Ctrl C3dM798E0 Ctrl C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Ctrl C4dM798E0 Ctrl mIgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Ctrl mIgGM798E0 Ctrl RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Ctrl RBCM798E0 Ctrl WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.Ctrl WGAM798E0 C3DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M798E0 C3dM798E0 C4DFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M798E0 C4dM798E0 mIgGFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M798E0 RBCFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.M798E0 WGAFlow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Acute, inflammatory conditions associated with dysregulated complement activation are characterized by significant increases in blood concentration of reactive oxygen species (ROS) and ATP. The mechanisms by which these molecules arise are not fully understood. In this study, using luminometric- and fluorescence-based methods, we show that ligation of glycophorin A (GPA) on human red blood cells (RBCs) results in a 2.1-fold, NADPH-oxidase-dependent increase in intracellular ROS that, in turn, trigger multiple downstream cascades leading to caspase-3 activation, ATP release, and increased band 3 phosphorylation. Functionally, using 2D microchannels to assess membrane deformability, GPS-ligated RBCs travel 33% slower than control RBCs, and lipid mobility was hindered by 10% using fluorescence recovery after photobleaching (FRAP). These outcomes were preventable by pretreating RBCs with cell-permeable ROS scavenger glutathione monoethyl ester (GSH-ME). Our results obtained in vitro using anti-GPA antibodies were validated using complement-altered RBCs isolated from control and septic patients. Our results suggest that during inflammatory conditions, circulating RBCs significantly contribute to capillary flow dysfunctions, and constitute an important but overlooked source of intravascular ROS and ATP, both critical mediators responsible for endothelial cell activation, microcirculation impairment, platelet activation, as well as long-term dysregulated adaptive and innate immune responses.
- Beth Israel Deaconess Medical Center United States
- University of Houston United States
- Harvard University United States
- Kyoto University Japan
sepsis, ATP, 2011-2015, caspase-3, Sepsis, complement
sepsis, ATP, 2011-2015, caspase-3, Sepsis, complement
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