This study presents the results of the first faunistic survey of millipedes (Diplopoda) conducted in the Chishui Alsophila National Nature Reserve, Guizhou Province, China. A total of eight species belonging to five families and four orders were identified. Two species are described as new to science: Hyleoglomeris erecta sp. nov. (Glomeridae) and Sellanucheza tumida sp. nov. (Paradoxosomatidae). Hyleoglomeris erecta sp. nov., characterized by its unique erect and not basally curved telopods, and Sellanucheza tumida sp. nov., distinguished by its conspicuously swollen gonopodal femorite. Additionally, one order (Julida), one family (Julidae), four genera (Niponia, Sellanucheza, Tylopus and Nepalmatoiulus) and four species (Niponia nodulosa, Kronopolites biagrilectus, Tylopus nigromarginatus, and Nepalmatoiulus sichuanensis) are recorded from Guizhou Province for the first time. This research provides essential baseline data for understanding the millipede biodiversity of the region.