Chrysosplenium ningqiangense (Saxifragaceae) is described as a new species discovered in Hanshuiyuan Forest Park, Ningqiang County, Shaanxi Province, China. Morphologically, it resembles C. serreanum , C. alternifolium and C. japonicum , but can be distinguished by its leaf shape, yellow and loose inflorescences, absence of stolons, bulbils or bulbs at the stem base, and reddish-brown seeds with a tessellate and papillate surface. Phylogenetic analyses, based on ITS and cpDNA sequences, indicate that C. ningqiangense clusters with C. hydrocotylifolium , C. guangxiense , C. macrophyllum and C. zhangjiajieense . Although these species differ in morphological characteristics, phylogenetic evidence confirms C. ningqiangense as a distinct species within the subgenus Gamosplenium .