Gentiana daofuensis , a new species of the family Gentianaceae, is described and illustrated here. Currently, this species is known only from Daofu County, Sichuan Province, China. Phylogenetic analyses based on chloroplast genome data indicate that it belongs to G. section Chondrophyllae s.l. Morphologically and genetically, it is closely related to G. crassuloides but can be clearly distinguished from the latter by its shorter calyx and pale blue-purple corolla with numerous dark blue short stripes or spots.