A new species of Gesneriaceae, Primulina acutiloba C.Xiong, J.X.Fu & F.Wen, from the limestone karsts of Northwest Guangxi, China, is described and illustrated. Morphologically, it resembles Pri. pingguoensis H.S.Ma & B.Pan and Pri. pseudoeburnea (D.Fang & W.T.Wang) Mich. Möller & A. Weber in corolla shape and coloration, particularly in the presence of purple stripes extending from the throat to the lobes. However, it can be readily distinguished by a combination of characters, including the shape of the leaf blades, bracts, and calyx lobes, the length of the peduncle and pistil, and the height of the disc. Currently, only a single population is known from the type locality, comprising approximately 60–80 mature individuals. Following the IUCN Red List Categories and Criteria, the species is provisionally assessed as Endangered (EN C2 b).