Rhizosphere soil, serving as the micro-ecological interface linking plant roots and the surrounding environment, is critical for the tea plant, as it harbors a rich diversity of microbial species that influence nutrient absorption and transformation in plant roots. During a fungal diversity survey of rhizosphere soil from tea plants, 10 fungal strains were isolated from soil samples collected in Guizhou, China. Based on morphological characteristics and multi-gene phylogenetic analysis, they were identified and proposed as three new species: Arthrographis guizhouensis sp. nov., Coniochaeta guizhouensis sp. nov., and Spiromyces sinensis sp. nov. In this study, morphological descriptions, illustrations, and molecular phylogenetic analyses of these three new species are presented.