﻿Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae)

descriptionPublicationkeyboard_double_arrow_right Article 17 Dec 2021 Italy Publisher:Pensoft PublishersJournal:Comparative Cytogenetics, volume 15, pages 507-525 (issn: 1993-0771, eissn: 1993-078X,

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Authors: Alessio Turco; Antonella Albano; Pietro Medagli; Robert P. Wagensommer; Saverio D’Emerico;

doi: 10.3897/compcytogen.v15.i4.75990

pmid: 35070135

pmc: PMC8709834

handle: 11587/471556 , 11586/381725

Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae)

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Abstract

In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time onOphrys tenthrediniferaWilldenow, 1805,Serapias vomeracea(Burman f., 1770) Briquet, 1910 andHimantoglossum hircinum(Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed onO. tenthrediniferausing Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescencein situhybridisation (FISH) with rDNA probes. We analysed two subspecies, i.e. O. tenthredinifera subsp. neglecta (Parlatore, 1860) E.G. Camus, 1908 and O. tenthredinifera subsp. grandiflora (Tenore, 1819) Kreutz, 2004 by the traditional Feulgen method and constructed the karyotype. The cytotaxonomic implications for both taxa are also discussed. InHimantoglossum hircinum, FISH and silver staining highlighted differences in the number of two rDNA families (35S and 5S) with respect toBarlia robertiana(Loiseleur-Deslongchamps, 1807) Greuter, 1967. In addition, fluorescencein situhybridisation was also applied to diploid (2n= 2x= 36) and triploid (2n= 3x= 54)Anacamptis morio(Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997. As far as we are aware, this is the first case of autotriploidy observed inA. morio.

Country

Italy

Related Organizations

University of Salento
Italy
University of Bari Aldo Moro
Italy

Keywords

Liliopsida, Karyotype, Asparagales, Anacampti, Anacamptis; C-banding; Cytotaxonomy; FISH; Himantoglossum; Karyotype; Ophrys; Serapias, QH426-470, Anacamptis, Biota, C-banding, Serapias, Ophrys, Tracheophyta, FISH, Genetics, Himantoglossum, Plantae, Orchidaceae, Cytotaxonomy, Ophry, Research Article

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