Research on sea spiders in China is relatively scarce and there is a severe shortage of regional basic data. In earlier studies, there was a lack of attention given to this small group and museums housed a large number of specimens that had not been identified.By re-examining the collections of sea spiders in the Marine Biological Sample Museum in the Third Institute of Oceanography of MNR (MBSM), 12 specimens were found to belong to five families, five genera and five species. Amongst them, four species were new records for Fujian, while Anoplodactylus tubiferus (Haswell, 1884) was a new record for China. Through reviewing previous studies, a checklist of sea spiders in Fujian was established and this study increased the number of recorded species from 10 to 14.