The aim of this report is to summarize the applicability of nanocomposites against clinically significant pathogen Salmonella entherica serotype holeraesius. The antibacterial effect of single SiO2, RGO nanoparticles and combination of Ag decorated RGO, SiO2, RGO and ZnTiO3 were examined. ZnTiO3 nanoparticles were prepared using nonhydrolitic sol-gel method. The various nanoparticles combinations were evaluated, dispersed in type I fibrillary collagen gel with concentration of 2.64 w% in different ratios with nanoparticles. The liophylization process was used to prepare nanocomposite material. Several nanocomposites as Coll: RGO, Coll: Ag/RGO, Ag/ SiO2/RGO, ZnTiO3/ SiO2/RGO, Coll: ZnTiO3 were tested on S. enterica. The formed sterile zones around the disc samples (diameter of 9.0 mm; thickness of 3 mm) were measured in mm (± 0.5). Some of the tested materials have shown antibacterial activity on S. enterica but the other have no effect. The research team relies on an innovative combination of qualities of different nanoparticles which aims synergy and high antibacterial activity with potential application in medical practice.