This report gives the research work carried out for design and analysis of MIMO antenna using two identical Microstrip slot radiators having enhanced isolation. The slot radiators offer compact size in order to accommodate other electronic components for reduction of volume of the wireless communication system. The defected ground structure is formed on the ground plane in between the antenna elements and feed lines to improve the isolation between them. The substrate used for constructing the antenna is FR-4 having the measurements of 26mm x 22mm x 0.8mm and it has the relative permittivity of 4.4. The printed microfilm strip etched on the opposite side of the substratum is used to couple the signal to each antenna. The HFSS software is used in this paper for designing the antenna and for checking the performance of the antenna. The -10dB bandwidth is 1.1GHz in the frequency range of 3.1 GHZ to 4.2GHz. The maximum isolation obtained after simulation is -23.1dB at 3.13GHz. The maximum gain of 2.26dB is obtained. Simulated radiation diagram of the designed antenna indicates that it is a good radiator for 5G applications in the sub 6GHz frequency band.