Herein presented, we demonstrate that a sensitive sensing/detection element was obtained from the laser treatment of a non-conducting flexible material exploiting laser machine, which can then potentially deploy as sensing element of a biosensor for possible usage in to sense and obtain the presence and quantity of the interested sample. The goal is to study and advance innovative means of fabricating a low-cost graphene sensor, employed as a Radio Frequency (RF) filter for disposable biomedical purposes. A material like Graphene can be fashioned by laser irradiation (Laser scribe) of Kapton tape implemented as a filter. The manufacture of the filter geometry was accomplished by means of a laser machine irradiating a Kapton tape on a chosen substrate (for this work a Flame Retardant 4 (FR-4)), by the application of the previous gotten parameters for the production. Various laser power values were employed for their manufacture and their corresponding conductivity was observed to range from 171 x10-6 S/mm to 279 x10-6 S/mm. The Raman spectrum results of the produced material has a D band peak at 1349.76 cm-1 , a G band of 1587.73 cm-1 and a 2D band peak of 2693.34 cm-1 . The ANSYS high-frequency structure simulator (HFSS) (for the Analysis of the System) simulation results signifies good outcomes, and opportunities to improve the material property are also being studied. Tests were also conducted by the utilization of a Vector Network Analyzer (VNA) to validating their feasibility of being deployed as the detection element of a biosensor, thus lending them the possibility to find implementation in disposable biological sensing.