This paper reviews the puzzles in modern neutron lifetime measurements and related unitarity issues in the CKM matrix. It is not a comprehensive and unbiased compilation of all historic data and studies, but rather a focus on compelling evidence leading to new physics. In particular, the largely overlooked nuances of different techniques applied in material and magnetic trap experiments are clarified. Further detailed analysis shows that the “beam” approach of neutron lifetime measurements is likely to give the “true” β-decay lifetime, while discrepancies in “bottle” measurements indicate new physics at play. The most feasible solution to these puzzles is a newly proposed ordinary-mirror neutron (n−n′) oscillation model under the framework of mirror matter theory. This phenomenological model is reviewed and introduced, and its explanations of the neutron lifetime anomaly and possible non-unitarity of the CKM matrix are presented. Most importantly, various new experimental proposals, especially lifetime measurements with small/narrow magnetic traps or under super-strong magnetic fields, are discussed in order to test the surprisingly large anomalous signals that are uniquely predicted by this new n−n′ oscillation model.