The accessibility of basic services in rural settlements in the South Moravian Region was evaluated; the accessibility by public transport was taken into account because the accessibility by individual car transport does not manifest any problems. The accessibility was calculated for 768 rural settlements, defined as spatially separate places which do not belong to municipalities with extended power. The time distance includes the time of the ride plus 8 min walking distance to and from the stop. The data was taken from the Integrated Transport System of the South Moravian Region, which ensures that each populated place in the region has to be connected by public transport at least six times on working days. The results show that for 99.8% of the inhabitants of the region, central places equipped with a post office, a basic school and a general practitioner’s office are accessible in a shorter time than 30 min. The accessibility of the services depends primarily on the characteristics of the settlement system and the physical conditions of the transport. In lowland areas, the share of settlements with comfortable accessibility is expressively higher than that of the highland and peripheral territories. The possible future improvement consists of the digitization of the services.