LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 29 Sep 2020 Spain English Publisher:MDPI AGJournal:Marine Drugs, volume 18, page 497 (eissn: 1660-3397,

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Authors: Marvin Wellkamp; Francisco García-Camacho; Lorena M. Durán-Riveroll; Jan Tebben; Urban Tillmann; Bernd Krock;

doi: 10.3390/md18100497

pmid: 33003497

pmc: PMC7601433

handle: 10835/8693

LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

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Abstract

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

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Spain

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Keywords

glycosylation, QH301-705.5, dinoflagellate, Article, Tandem Mass Spectrometry, Polyketides, ichthyotoxin, Dinoflagellida, harmful algae, Biology (General), phycotoxin, Chromatography, Liquid

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