Background: Anemia is one of the common complications of diabetes mellitus, and their risk in diabetic patients is estimated to be 2 – 3 times higher than that of patients without diabetes. Therefore, having a major impact on the overall health and survival of diabetes patients. Globally, the prevalence of concurrent anemia and diabetes mellitus ranges from 14 – 45% in various ethnic populations worldwide. Objectives: The aim of this study was to determine the prevalence of anemia among type II diabetic patients. Methodology: This is a cross-sectional laboratory-based study conducted in Alkhair Medical Center, Wad Medani, Gezira State, Sudan during March to December 2020 among 100 type II diabetic patients ((32 males and 68 females) their ages ranged between 30 and 90 years with mean (52.26 ± 13.58 years). Three ml of venous blood samples were collected from all participants in K3EDTA containers. A thin blood film was prepared and stained immediately. The RBCs parameters were performed using Sysmex XP-300 Automated Hematology Analyzer. SPSS computer program (v 25.0) was used for data analysis. Results: The study results showed that the prevalence of anemia among patients with type 2 Diabetes Mellitus were 7 (7 %). 6 (85.7 %) patients had mild anemia, and one (14.3 %) had moderate anemia according to clinical degree of anemia based on hemoglobin level. 4 (57.14 %) patients had microcytic hypochromic anemia, and 2 (28.57 %) patients had normocytic normochromic anemia and one (14.29 %) patients had macrocytic anemia based on RBCs indices and morphology. The prevalence of anemia is more in female (7.35 %) than male (6.25 %). Conclusion: The study concluded that anemia among patients with type II Diabetes Mellitus was 7 %, mostly mild and microcytic hypochromic anemia; so improve their nutrition status among diabetic patients may help in reducing the anima during diabetes.