The Topic deal with biomedical, biological, medicinal, as well as economic fea- tures of the original substances as well as with their chemical components. The current study is one such effort to assess the physicochemical phyto- chemical studies and acute toxicity studies of extract of roots of Passiflora foetida (passiflorine). To study the antiarthritic activity of extract of roots Passiflora foetida . A present work demonstrated the estimation of serum SGOT (UV-Kinetic Method) and serum alkaline phosphate hydrolysis p-Nitro phenyl phosphate within presence of an oxidizing agent Mg +2 . All these inter- action has been assessed just like directly absorbencies proportionate to an ALP action. The Physicochemical Standards, Preliminary Phytochemical Stud- ies and Haematological Parameter Express the values in mean ± SEM, (n=6), it when compared with the control, *P<0.05, **P<0.01, ***P<0.001 one way ANOVA accompanied through Dunnett’s t-Test. However, we perform the Effect of ethanolic extract of whole plant of Passiflora foetida on mean changes in paw volume as well as Biochemical parameters evaluation of ethanolic extract of root of Passiflora foetida .