Forcepsioneura Lencioni, 1999 is a small genus of eight forest-dependent damselfly species endemic to the Brazilian Atlantic Forest domain. Some of its species are difficult to identify due to their strong morphological similarities. Thus, the use of DNA sequences for taxonomic purposes is warranted. This study examined the diversity among mitochondrial COI and 16S and nuclear PRMT markers in Forcepsioneura, identified discrete evolutionary units based on morphological and molecular characters, and described two new species using an integrative approach to propose species-level hypotheses. The first molecular phylogeny of Forcepsioneura species, including seven of the 10 valid species, is presented. Forcepsioneura gabriela sp.n. and Forcepsioneura janeae sp.n. are described and illustrated based on males. Forcepsioneura gabriela sp.n. is closely related to F. garrisoni Lencioni, 1999 and F. regua Pinto & Kompier, 2018 and was included in the light blue group, but was recovered with high K2P COI divergence values relative to F. garrisoni. PRMT and ribosomal 16S rDNA sequences were too conservative to distinguish this new species from others of the light blue group. Nevertheless, F. gabriela sp.n. can be distinguished from other Forcepsioneura by its coloration and shape and length of the ventrobasal process of cercus and MP vein. On the other hand, we were unable to get COI sequences for F. janeae sp.n., but morphological diagnostic characters, such as, coloration and shape of the posterior lobe of the prothorax and ventrobasal process of cercus supported its proposal as a new species. A concatenated Bayesian analysis of all markers supported the monophyly of both Forcepsioneura and the light blue group of species. This study affirmed the value of COI sequence variation for species-level studies but did not support the use of PRMT and 16S for this group of damselflies, as there was very little interspecific variation between some closely related species.