Supporting of a physiologically relevant cellular micro-environment is currently a grand challenge in the design of tissue-engineering grafts based on biocompatible and biodegradable polymeric materials. The aim of this research was to develop a new technique of a fibrous polycaprolactone-based scaffold modification with epidermal growth factor (EGF) and assessment of its effect on scaffold properties and proliferative activity of epithelial cells in vitro. Fibrous scaffolds from EGF-functionalized polycaprolactone has received by the emulsion electrospinning method. Prolonged yield of EGF upon the material destruction and its biological effect on the MCF7 cell line proliferation have been estimated using ELISA and iCEL-Ligence real-time cell analysis for respectively.