Background:Thrombocytopenia have become a common hematological disorder defined by the reduction of platelet count and is often accompanied by immune dysregulation. This study hasbeen conducted to investigate blood markers (RBC count, hemoglobin, packed cell volume, mean corpuscular hemoglobin) and immune markers (interleukin-6, tumor necrosis factor-alpha, interleukin-10) in patients with thrombocytopenia. Methods: A hospital-based case-control study in Al-Husseuni Hospital and Kerbala City, Iraq between June 2025 and December 2025. The study included a total of 120 subjects, including 58 patients with thrombocytopenia and 62 healthy controls. Blood markers were measured using an automated hematological analyzer, and serum cytokines were quantified by ELISA.