Ayurveda is a holistic science that emphasizes preserving and promoting the fitness of healthy individuals, besides providing a method for the treatment of diseases. In addition, sufficient infrastructure, human resources, and unique modalities for disease prevention, wellness, and management are available. In present scenario Ayush System is growing day by day with support of government with increase in fundings from INR 691 crore to INR 3050 crore over the last seven years. The Ayush systems can contribute significantly to the One Health approach, which addresses the interconnectedness of human, animal, and environmental health. For this purpose it is need of hour to integrate the Ayush system and other related system to provide the integration of particular areas which can be helpful to fulfil the approach of one health and future perspectives on the role of Ayush in One Health. Ayush system have the he unique principals related to preventive measures, such as daily and seasonal regimens and the use of rejuvenating herbs and Achar rasayanas as well as effective therapeutic interventions for the number of health issues for the contribution in one health. Ayurveda also addresses environmental, animal, and plant health issues and promotes the creation of a healthy ecosystem between humans, animals, and the environment. In addition, Ayush can play a role in addressing zoonotic diseases, noncommunicable diseases, antimicrobial resistance, food safety and health of the elderly, children, and environment. To fully utilize the potential of Ayush in the One Health approach, these systems should be integrated into the healthcare infrastructure and network. Furthermore, initiation of collaborative projects, focused research, training and sensitization of Ayush human resources, and promotion of Ayush‑based lifestyles and practices are some of the critical steps required to be taken.