The fish fauna of urban streams is still poorly known, it difficult to assess the effects of urbanization expansion on fish species composition, for this reason the aim of this study was to provide a checklist of species that compose the ichthyofauna of six urban streams, tributaries to the Vermelho River, upper Paraguay River Basin, Rondonópolis, Mato Grosso State, Brazil. The samples were performed with seine nets on a spatial gradient of 75 m, and with sieves for a period of 15 minutes in each site. A total of 56 species belonging to five orders, Characiformes, Siluriformes, Gymnotiformes, Cyprinodontiformes, and Perciformes, 21 families and 44 genera were sampled. The most common species were Astyanax asuncionensis Géry, 1972, Astyanax abramis (Jenyns, 1842), Odontostilbe pequira (Steindachner, 1882), Odontostilbe paraguayensis Eigenmann & Kennedy, 1903, Characidium zebra Eigenmann, 1909 and Hypostomus sp. This checklist brings additional knowledge on fish that inhabit tributaries to the major rivers of northern Pantanal.