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Split-QF system for fine-tuned transgene expression in Drosophila

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type , Preprint 23 Oct 2018 United Kingdom Publisher:openRxivJournal:Genetics, volume 212, pages 53-63 (eissn: 1943-2631, Copyright policy )Funded by:UKRI | Homeostatic control of ne..., NIH | Bloomington Drosophila St..., WT | Establishment of a Drosop... +1 projects
Authors: Olena Riabinina; Samuel W. Vernon; Barry J. Dickson; Richard A. Baines;

doi: 10.1101/451146 , 10.1534/genetics.119.302034

pmid: 30862621

pmc: PMC6499530

Split-QF system for fine-tuned transgene expression in Drosophila

Abstract

The Q-system is a binary expression system that works well across species. Here we report the development of a split-QF system that drives strong expression, is repressible by QS and inducible by quinic acid. The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines for advanced intersectional experiments, thus greatly expanding the range of possible anatomical, physiological and behavioural assays in Drosophila.

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Keywords

Male, Serine Endopeptidases, Quinic Acid, Gene Expression, quinic acid, Investigations, Animals, Genetically Modified, Bacterial Proteins, Genetic Techniques, LexA Repressor Protein, Animals, Drosophila Proteins, split-LexA, Drosophila, Female, split-GAL4, Transgenes, q-system, Transcription Factors

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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OpenAIRE UsageCountsViews provided by UsageCounts
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