The Taxonomic Collections Center of the Federal University of Minas Gerais (CCT-UFMG) has 223,653 insects catalogued, of which about 6,500 are beetles (Coleoptera). Most insects in the collection are from the southeastern region of Brazil. The collection also includes specimens from other regions and from other countries. A checklist of the flat-faced longhorn beetles (Cerambycidae, Lamiinae) deposited in the CCT-UFMG is presented. The available exemplars were identified using the literature or direct comparison with individuals from other scientific collections. The CCT-UFMG contains 254 specimens of Lamiinae that were identified to species level, comprising 100 species from 63 genera. This checklist includes a new country record of Aerenicopsis championi Bates, 1885 for Brazil and the following 26 new records for the state of Minas Gerais (Brazil): Apagomera bravoi Galileo & Martins, 2009; Atrypanius conspersus (Germar, 1824); Callia axillaris (Dalman, 1823); Callia xanthomera Redtenbacher, 1867; Cacostola acuticauda Marinoni & Martins, 1982; Cipriscola fasciata (Thomson, 1860); Colobothea centralis Monné, 1993; Colobothea rubroornata Zajciw, 1962; Desmiphora ornata Bates, 1866; Estola assimilis Breuning, 1940; Eupromerella griseofasciata (Fuchs, 1959); Hedypathes monachus (Erichson in Schomburg, 1848); Hesychotypa subfasciata Dillon & Dillon, 1945; Hippopsis pubiventris Galileo & Martins, 1988; Ischioderes oncideroides Dillon & Dillon, 1945; Laticranium mandibulare Lane, 1959; Lesbates caviunas (Dillon & Dillon, 1949); Nealcidion silvai Monné & Delfino, 1986; Oncideres alicei Lane, 1977; Oreodera curiosa Galileo & Martins, 2007; Phoebe phoebe (Lepeletier & Audinet-Serville, 1825); Polyrhaphis olivieri Thomson, 1865; Psapharochrus geminus Galileo & Martins, 2012; Psapharochrus nigricans (Lameere, 1885); Steirastoma breve (Sulzer, 1776); Tapeina rudifrons Marinoni, 1972. Photographs of specimens that represent new distribution records and a map showing the distribution of the species of Lamiinae present in the collection are provided.