Diagnoses and notes on biology and distribution of Japanese species of the suberis and angulifasciella groups of the genus Ectoedemia Busck, 1907 are given. Two species, E. chasanella Puplesis, 1984 and E. ortiva Rocienė & Stonis, 2013, are reported for the first time from Japan and their morphology is redescribed. In addition, we provide molecular identification of the two species and their relatives based on COI barcode sequences and the nuclear gene (EF1-α) sequences. The female of E. chasanella is described for the first time. We confirm that E. chasanella utilizes Quercus species (Fagaceae) as its hostplant: Q. dentata, Q. serrata, Q. crispula, and Q. acutissima. Ectoedemia ortiva was formerly placed in the suberis group, and its host plant was unknown. We move this species to the angulifasciella group and provide evidence that it utilizes Ulmus sp. (Ulmaceae) as its host plant.