Pluteus lauracearum, a new species of Pluteus sect. Hispidoderma, is described and illustrated based on macro- and micromorphological data and phylogenetic analyses of the nuclear rDNA sequences of the ITS1-5.8S-ITS2 region. Morphologically, this novel species is characterized by small basidiomata having whitish to dark brown pileus colour, with finely to distinctly granulose surface, a white and translucently striate pileus margin, and mostly narrowly fusiform to lageniform pleurocystidia with long narrow necks and a distinctly subcapitate to capitate apex. This new species is described based on collections made in Turkey (Kuşadası) and Portugal (Madeira), where they were growing on well-rotten wood in thermophilic Laurus forests. Pluteus lauracearum is compared with taxa in Pluteus sect. Hispidoderma that are morphologically similar and/or phylogenetically related.